3D2S
Crystal structure of MBNL1 tandem zinc finger 3 and 4 domain in complex with CGCUGU RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.421, 56.899, 58.222 |
| Unit cell angles | 90.00, 108.81, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.700 |
| R-factor | 0.2263 |
| Rwork | 0.224 |
| R-free | 0.25984 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | MBNL1 ZnF3/4 structure |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.631 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 38651 | |
| <I/σ(I)> | 17.7 | 3.6 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.5 | 277 | 25% PEG 3350, 0.1M Bis-Tris, pH 5.5, VAPOR DIFFUSION, temperature 277K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 3350 | ||
| 2 | 1 | 1 | Bis-Tris | ||
| 3 | 1 | 1 | H2O | ||
| 4 | 1 | 2 | PEG 3350 | ||
| 5 | 1 | 2 | Bis-Tris | ||
| 6 | 1 | 2 | H2O |
