3D2Q
Crystal structure of MBNL1 tandem zinc finger 3 and 4 domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.2827 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 29.166, 80.459, 55.357 |
| Unit cell angles | 90.00, 101.76, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.500 |
| R-factor | 0.19822 |
| Rwork | 0.197 |
| R-free | 0.21748 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.445 | |
| Number of reflections | 38947 | |
| <I/σ(I)> | 3.4 | |
| Completeness [%] | 97.7 | 95.7 |
| Redundancy | 3.2 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.8 | 277 | 18% PEG 3350, 0.1M Mg acetate, 0.2 M sodium fluoride, pH 6.8, VAPOR DIFFUSION, temperature 277K |
