3D2F

Crystal structure of a complex of Sse1p and Hsp70

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	CCD
Collection date	2007-05-03
Detector	MARRESEARCH
Wavelength(s)	0.9780
Spacegroup name	P 21 21 21
Unit cell lengths	129.500, 141.902, 151.680
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.300
R-factor	0.2
Rwork	0.197
R-free	0.24400
Starting model (for MR)	3d2e
RMSD bond length	0.014
RMSD bond angle	1.430
Data reduction software	MOSFLM
Data scaling software	SCALA (3.2.25)
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	103.695	75.840	2.420
High resolution limit [Å]	2.300	7.270	2.300
Rmerge	0.073	0.030	0.506
Total number of observations		14778	62222
Number of reflections	124395
<I/σ(I)>	7.6	18.8	1.5
Completeness [%]	100.0	99.3	100
Redundancy	3.7	3.5	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8	277	15 % (w/v) polyethyleneglycol-6000, 100 mM Tris-HCl pH 8.0, 10 mM DTT, VAPOR DIFFUSION, temperature 277K