3D2E
Crystal structure of a complex of Sse1p and Hsp70, Selenomethionine-labeled crystals
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-05-03 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9719, 0.9792, 0.9795 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 129.840, 141.649, 150.516 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.350 |
| R-factor | 0.213 |
| Rwork | 0.210 |
| R-free | 0.26200 |
| Structure solution method | MAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.430 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.25) |
| Phasing software | SHELX |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 103.142 | 80.850 | 2.480 |
| High resolution limit [Å] | 2.350 | 7.430 | 2.350 |
| Rmerge | 0.058 | 0.022 | 0.472 |
| Total number of observations | 13808 | 56550 | |
| Number of reflections | 115684 | ||
| <I/σ(I)> | 9.8 | 21.4 | 1.6 |
| Completeness [%] | 99.9 | 98.3 | 99.7 |
| Redundancy | 3.7 | 3.6 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 277 | 17 % (w/v) polyethyleneglycol-6000, 50 mM KCl, 100 mM HEPES-KOH pH 7.5, vapor diffusion, temperature 277K |
