3D1P
Atomic resolution structure of uncharacterized protein from Saccharomyces cerevisiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-31 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.6525 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 58.343, 37.429, 52.198 |
| Unit cell angles | 90.00, 108.86, 90.00 |
Refinement procedure
| Resolution | 30.000 - 0.980 |
| R-factor | 0.10897 |
| Rwork | 0.108 |
| R-free | 0.12369 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.723 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.000 |
| High resolution limit [Å] | 0.980 | 0.980 |
| Rmerge | 0.062 | 0.650 |
| Number of reflections | 59810 | |
| <I/σ(I)> | 20 | 1.9 |
| Completeness [%] | 98.3 | 95.5 |
| Redundancy | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.3 | 291 | 2.4 M Ammonium sulfate, pH 8.3, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
