3D1H
Structure of the PTP-Like Phytase Expressed by Selenomonas Ruminantium at an Ionic Strength of 500 mM
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-10-20 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.1158 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 45.858, 137.174, 79.839 |
Unit cell angles | 90.00, 102.97, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.100 |
R-factor | 0.186 |
Rwork | 0.184 |
R-free | 0.21400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2b4p |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.063 | 0.207 |
Number of reflections | 46736 | |
<I/σ(I)> | 16.6 | 3.2 |
Completeness [%] | 91.0 | 56.4 |
Redundancy | 2 | 1.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.8 | 293 | 10% P8K, 450 MM NACL, 50 MM NA-ACETATE, pH 4.8, VAPOR DIFFUSION, SITTING DROP, temperature 293K |