3D1B
Tetragonal crystal structure of Tas3 C-terminal alpha motif
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9A |
| Synchrotron site | NSLS |
| Beamline | X9A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-05-04 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9772 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 64.985, 64.985, 169.491 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.700 |
| Rwork | 0.207 |
| R-free | 0.23700 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.135 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.700 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.076 | 0.608 |
| Number of reflections | 39086 | |
| <I/σ(I)> | 39.4 | 4.1 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 15.8 | 16 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 12% PEG4000, 0.1 M Tris 7.5, 0.2 M KCl and 50 mM MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
