3D1A
Crystal Structure Determination of Goat Hemoglobin at 2.61 Angstrom Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-03-06 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54179 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.402, 67.138, 153.454 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.780 - 2.610 |
| R-factor | 0.2208 |
| Rwork | 0.217 |
| R-free | 0.25745 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g08 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.272 |
| Data reduction software | AUTOMAR |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 14.960 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.054 | |
| Number of reflections | 18937 | |
| <I/σ(I)> | 7.6 | 2 |
| Redundancy | 3.24 | 2.88 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 2mM PEG 3350, 1M NaCl, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
