3D0U
Crystal Structure of Lysine Riboswitch Bound to Lysine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-01-28 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.1050 |
| Spacegroup name | P 32 |
| Unit cell lengths | 119.823, 119.823, 58.744 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.619 - 2.800 |
| R-factor | 0.184 |
| Rwork | 0.182 |
| R-free | 0.20900 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.087 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.084 | 0.403 |
| Number of reflections | 24657 | |
| <I/σ(I)> | 13.5 | |
| Completeness [%] | 95.6 | 61.4 |
| Redundancy | 5 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 303 | 2 M Li2SO4, 5 mM MgCl2, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 303K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Li2SO4 | ||
| 2 | 1 | 1 | MgCl2 | ||
| 3 | 1 | 2 | Li2SO4 | ||
| 4 | 1 | 2 | MgCl2 |
