3D0T

Structure of the BNB domain of the Hsp70 cochaperone Bag2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	95
Detector technology	CCD
Collection date	2007-02-10
Detector	NOIR-1
Wavelength(s)	0.96410908, 0.99505962
Spacegroup name	H 3 2
Unit cell lengths	104.680, 104.680, 164.300
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.690 - 2.550
Rwork	0.253
R-free	0.28400
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.200
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	PHENIX
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.340	2.640
High resolution limit [Å]	2.550	2.550
Rmerge	0.059	0.242
Total number of observations		6731
Number of reflections	11398
<I/σ(I)>	14.5	5.7
Completeness [%]	98.8	99.9
Redundancy	5.96	5.92

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.6	293	35% PEG 400, 0.1M Bis-Tris pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K