3D0S
cAMP receptor protein from m.tuberculosis, cAMP-free form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-30 |
| Detector | ADSC |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.796, 83.803, 98.389 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 16.000 - 2.000 |
| R-factor | 0.21585 |
| Rwork | 0.212 |
| R-free | 0.28784 |
| Structure solution method | SAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.667 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | RESOLVE (2.13) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 20.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 4.300 | 2.000 |
| Rmerge | 0.078 | 0.052 | 0.333 |
| Number of reflections | 29026 | ||
| <I/σ(I)> | 13.9 | ||
| Completeness [%] | 95.2 | 98 | 93.7 |
| Redundancy | 6.2 | 6.2 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 20 mg/mL ptn, 80 mM NaCl, l4% PEG4K, 8% IPA, 0.1M Hepes, pH 7.5, vapor diffusion, hanging drop, temperature 298K |
