3D0O
Crystal structure of Lactate Dehydrogenase from Staphylococcus Aureus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Collection date | 2008-04-28 |
Wavelength(s) | 1.0809 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 131.765, 74.503, 96.306 |
Unit cell angles | 90.00, 129.07, 90.00 |
Refinement procedure
Resolution | 29.370 - 1.800 |
R-factor | 0.172 |
Rwork | 0.170 |
R-free | 0.20300 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.017 |
RMSD bond angle | 1.524 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.4.0069) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.760 |
High resolution limit [Å] | 1.700 | 3.660 | 1.700 |
Rmerge | 0.084 | 0.070 | 0.259 |
Number of reflections | 75432 | ||
<I/σ(I)> | 7.7 | ||
Completeness [%] | 94.6 | 97.8 | 67.1 |
Redundancy | 2.8 | 3.1 | 1.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |