3D0M
X-ray structures of the (GUGGUCUGAUGAGGCC) RNA duplex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X13 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-01-26 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.8020 |
Spacegroup name | H 3 2 |
Unit cell lengths | 46.653, 46.653, 126.397 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 2.000 |
Rwork | 0.295 |
R-free | 0.36100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | another crystal form (pdb id 3czw) |
RMSD bond length | 0.020 |
RMSD bond angle | 3.978 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.030 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 3434 | |
<I/σ(I)> | 37 | 4.7 |
Completeness [%] | 99.8 | 100 |
Redundancy | 10.6 | 8.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 1.7 M ammonium sulphate, 50 mM cacodylate buffer, 15 mM magnesium acetate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | (NH4)2SO4 | ||
2 | 1 | 1 | magnesium acetate | ||
3 | 1 | 1 | cacodylate | ||
4 | 1 | 2 | (NH4)2SO4 | ||
5 | 1 | 2 | magnesium acetate |