3D0K
Crystal structure of the LpqC, poly(3-hydroxybutyrate) depolymerase from Bordetella parapertussis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-23 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97920 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.879, 83.710, 90.792 |
| Unit cell angles | 90.00, 92.68, 90.00 |
Refinement procedure
| Resolution | 41.830 - 1.830 |
| R-factor | 0.166 |
| Rwork | 0.164 |
| R-free | 0.20900 |
| Structure solution method | SAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.639 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.830 | 1.860 |
| High resolution limit [Å] | 1.830 | 1.830 |
| Number of reflections | 54978 | |
| <I/σ(I)> | 8.5 | 2 |
| Completeness [%] | 99.8 | 99.7 |
| Redundancy | 4.5 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 1.6 M Ammonium sulfate, 0.1 M MES pH 6.5, 10% Dioxane, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
