3D01
Crystal structure of the protein Atu1372 with unknown function from Agrobacterium tumefaciens
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-12-04 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9794, 0.9796 |
Spacegroup name | P 1 |
Unit cell lengths | 93.623, 93.600, 93.628 |
Unit cell angles | 68.20, 67.87, 68.14 |
Refinement procedure
Resolution | 46.130 - 1.700 |
R-factor | 0.18066 |
Rwork | 0.179 |
R-free | 0.21411 |
Structure solution method | MAD |
RMSD bond length | 0.016 |
RMSD bond angle | 1.447 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 83.620 | 1.745 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.084 | 0.605 |
Number of reflections | 266266 | |
<I/σ(I)> | 20.33 | 1.5 |
Completeness [%] | 97.0 | 92.08 |
Redundancy | 5 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 289 | 0.1M Bis-tris pH 5.5, 0.2M NH4(OAC), 25% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 289K |