3D00

Crystal structure of a tungsten formylmethanofuran dehydrogenase subunit e (fmde)-like protein (syn_00638) from syntrophus aciditrophicus at 1.90 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-04-03
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91837,0.97817
Spacegroup name	P 41 21 2
Unit cell lengths	54.356, 54.356, 136.719
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.386 - 1.900
R-factor	0.235
Rwork	0.233
R-free	0.26800
Structure solution method	MAD
RMSD bond length	0.019
RMSD bond angle	1.604
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHELX
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.386	29.386	1.950
High resolution limit [Å]	1.900	8.500	1.900
Rmerge	0.078	0.011	0.011
Total number of observations		1244	8626
Number of reflections	16955
<I/σ(I)>	6.8	16.4	0.7
Completeness [%]	99.9	95.9	99.9
Redundancy	7	5.2	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	277	0.01M NiCl2, 20.0% PEG MME 2000, 0.1M Tris-HCl pH 8.5, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K