3CZQ
Crystal structure of putative polyphosphate kinase 2 from Sinorhizobium meliloti
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-24 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97920 |
| Spacegroup name | P 1 |
| Unit cell lengths | 59.609, 71.672, 89.471 |
| Unit cell angles | 75.86, 85.97, 65.39 |
Refinement procedure
| Resolution | 34.700 - 2.230 |
| R-factor | 0.18968 |
| Rwork | 0.187 |
| R-free | 0.24030 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.431 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.700 | 2.310 |
| High resolution limit [Å] | 2.230 | 2.230 |
| Rmerge | 0.066 | 0.381 |
| Number of reflections | 56568 | |
| <I/σ(I)> | 15.2 | 1.99 |
| Completeness [%] | 89.4 | 46.8 |
| Redundancy | 3.6 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 0.1 M Sodium formate, 0.1 M Lithium sulfate, 0.1 M Bis-tris buffer, 0.3 M NDSB-211, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
