3CZG
Crystal Structure Analysis of Sucrose hydrolase (SUH)-glucose complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 4A |
Synchrotron site | PAL/PLS |
Beamline | 4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-03-20 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.99998 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 96.800, 119.200, 55.900 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.800 |
Rwork | 0.193 |
R-free | 0.23000 |
RMSD bond length | 0.005 |
RMSD bond angle | 1.222 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.860 |
High resolution limit [Å] | 1.800 | 3.880 | 1.800 |
Rmerge | 0.145 | 0.123 | 0.449 |
Rmeas | 0.145 | ||
Total number of observations | 356950 | ||
Number of reflections | 59781 | ||
<I/σ(I)> | 9 | ||
Completeness [%] | 97.8 | 99.4 | 78.9 |
Redundancy | 6 | 5.7 | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295 | 0.1M HEPES, 0.2M calcium acetate, 16% PEG 3000, 5mM DTT with 0.1M guanidine chloride, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K |