3CZG
Crystal Structure Analysis of Sucrose hydrolase (SUH)-glucose complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-20 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.99998 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 96.800, 119.200, 55.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.800 |
| Rwork | 0.193 |
| R-free | 0.23000 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.222 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 3.880 | 1.800 |
| Rmerge | 0.145 | 0.123 | 0.449 |
| Rmeas | 0.145 | ||
| Total number of observations | 356950 | ||
| Number of reflections | 59781 | ||
| <I/σ(I)> | 9 | ||
| Completeness [%] | 97.8 | 99.4 | 78.9 |
| Redundancy | 6 | 5.7 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295 | 0.1M HEPES, 0.2M calcium acetate, 16% PEG 3000, 5mM DTT with 0.1M guanidine chloride, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
