3CZ7
Molecular Basis for the Autoregulation of the Protein Acetyl Transferase Rtt109
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X3A |
| Synchrotron site | NSLS |
| Beamline | X3A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-09-20 |
| Detector | MAR CCD 165 mm |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 120.782, 68.886, 55.241 |
| Unit cell angles | 90.00, 106.65, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| R-factor | 0.158 |
| Rwork | 0.156 |
| R-free | 0.18900 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.545 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.000 |
| Number of reflections | 55305 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.4 | 294 | 12% (w/v) PEG 4,000, 1 mM ZnCl2, and 100 mM Sodium Acetate, pH 4.4, temperature 294K, VAPOR DIFFUSION, HANGING DROP |
