3CYP

The crystal structure of the C-terminal domain of Helicobacter pylori MotB (residues 125-256).

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	CCD
Collection date	2007-05-13
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9
Spacegroup name	P 1 21 1
Unit cell lengths	50.816, 89.479, 66.322
Unit cell angles	90.00, 112.55, 90.00

Refinement procedure

Resolution	50.572 - 1.600
R-factor	0.188
Rwork	0.185
R-free	0.22700
RMSD bond length	0.014
RMSD bond angle	1.494
Data reduction software	MOSFLM
Data scaling software	SCALA (3.2.25)
Phasing software	SHELXS
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	61.200	50.570	1.690
High resolution limit [Å]	1.600	5.060	1.600
Rmerge	0.071	0.048	0.398
Total number of observations		7362	39340
Number of reflections	71913
<I/σ(I)>	5.9	13.2	1.9
Completeness [%]	99.6	89	100
Redundancy	3.8	3.5	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.4	293	100 mM Tris/HCl, 16-18% PEG 3350, 200 mM sodium tartrate, pH 6.4, VAPOR DIFFUSION, SITTING DROP, temperature 293K