3CYP
The crystal structure of the C-terminal domain of Helicobacter pylori MotB (residues 125-256).
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-05-13 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 50.816, 89.479, 66.322 |
Unit cell angles | 90.00, 112.55, 90.00 |
Refinement procedure
Resolution | 50.572 - 1.600 |
R-factor | 0.188 |
Rwork | 0.185 |
R-free | 0.22700 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.494 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.2.25) |
Phasing software | SHELXS |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 61.200 | 50.570 | 1.690 |
High resolution limit [Å] | 1.600 | 5.060 | 1.600 |
Rmerge | 0.071 | 0.048 | 0.398 |
Total number of observations | 7362 | 39340 | |
Number of reflections | 71913 | ||
<I/σ(I)> | 5.9 | 13.2 | 1.9 |
Completeness [%] | 99.6 | 89 | 100 |
Redundancy | 3.8 | 3.5 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.4 | 293 | 100 mM Tris/HCl, 16-18% PEG 3350, 200 mM sodium tartrate, pH 6.4, VAPOR DIFFUSION, SITTING DROP, temperature 293K |