3CYN
The structure of human GPX8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-10 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 90.688, 120.854, 75.603 |
| Unit cell angles | 90.00, 119.38, 90.00 |
Refinement procedure
| Resolution | 44.990 - 2.000 |
| R-factor | 0.17217 |
| Rwork | 0.171 |
| R-free | 0.19890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2p31 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.217 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.990 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.092 | 0.394 |
| Number of reflections | 47649 | |
| <I/σ(I)> | 8.9 | 3.1 |
| Completeness [%] | 99.7 | 99.2 |
| Redundancy | 4.1 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 2 M ammonium sulfate, 1.5 % PEG 400, 0.1 M hepes, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
