3CY5
Crystal structure determination of buffalo (Bubalus bubalis) hemoglobin at 2 angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-11 |
| Detector | BRUKER SMART 6000 |
| Wavelength(s) | 1.54179 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.064, 74.677, 110.224 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.220 - 2.000 |
| R-factor | 0.20069 |
| Rwork | 0.193 |
| R-free | 0.26969 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g08 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.210 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.295 | |
| Number of reflections | 34558 | |
| <I/σ(I)> | 1 | |
| Redundancy | 2.91 | 2.76 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 293 | 50% PEG 3350, 50mM phosphate buffer pH 6.7, 1M Nacl, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
