3CXQ

Crystal structure of human glucosamine 6-phosphate N-acetyltransferase 1 bound to GlcN6P

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER AXS MICROSTAR
Temperature [K]	100
Detector technology	CCD
Collection date	2008-03-03
Detector	BRUKER SMART 6000
Wavelength(s)	1.5418
Spacegroup name	P 43 21 2
Unit cell lengths	54.136, 54.136, 138.638
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.300
R-factor	0.209
Rwork	0.207
R-free	0.25500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2huz
RMSD bond length	0.009
RMSD bond angle	1.139
Data scaling software	LSCALE
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	54.348	54.350	2.460
High resolution limit [Å]	2.300	4.950	2.300
Number of reflections	8884
<I/σ(I)>	9.2	16.4	1.3
Completeness [%]	90.4
Redundancy	6.04

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	289	0.2M Na formate, 25% w/v PEG 3350, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K