3CXP

Crystal structure of human glucosamine 6-phosphate N-acetyltransferase 1 mutant E156A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BSRF BEAMLINE 3W1A
Synchrotron site	BSRF
Beamline	3W1A
Temperature [K]	100
Detector technology	CCD
Collection date	2008-02-23
Detector	MAR CCD 165 mm
Wavelength(s)	1.0001
Spacegroup name	P 43 21 2
Unit cell lengths	51.340, 51.340, 142.610
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.300 - 2.010
R-factor	0.2
Rwork	0.199
R-free	0.23200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2huz
RMSD bond length	0.007
RMSD bond angle	0.967
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.000		2.120
High resolution limit [Å]	2.000	5.770	2.000
Rmerge	0.078	0.031	0.414
Number of reflections	13549	654	2103
<I/σ(I)>	42.22	99	10.8
Completeness [%]	99.6	95.8	99.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.9	289	0.1M K Thiocyanate, 30% w/v PEG MME 2000, pH 6.9, VAPOR DIFFUSION, SITTING DROP, temperature 289K