3CXG
Crystal structure of Plasmodium falciparum thioredoxin, PFI0790w
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-09-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97900 |
| Spacegroup name | P 2 3 |
| Unit cell lengths | 97.455, 97.455, 97.455 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.380 - 2.000 |
| R-factor | 0.195 |
| Rwork | 0.192 |
| R-free | 0.24800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.028 |
| RMSD bond angle | 1.858 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.051 | 0.245 |
| Number of reflections | 21155 | |
| <I/σ(I)> | 13.5 | 2.09 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 11.7 | 9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 2.0 M Ammonium sulfate, 0.2 M Sodium acetate, 0.1 M Hepes pH 7.5, 5% MPD, 15% Glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
