3CX9
Crystal Structure of Human serum albumin complexed with Myristic acid and lysophosphatidylethanolamine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-01-01 |
| Detector | BRUKER SMART 2000 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 183.888, 39.015, 95.633 |
| Unit cell angles | 90.00, 104.69, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.800 |
| R-factor | 0.2234 |
| Rwork | 0.219 |
| R-free | 0.29527 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1n5u |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.521 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.873 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.057 | 0.356 |
| Number of reflections | 15244 | |
| Completeness [%] | 79.1 | 85.7 |
| Redundancy | 2 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 35% PEG 3350, 85mM potassium phosphate, 5mM sodium azide, pH7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |
