3CX3
Crystal structure Analysis of the Streptococcus pneumoniae AdcAII protein
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-19 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.971968, 0.979648, 0.979896 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.760, 63.260, 151.660 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.900 - 2.400 |
| R-factor | 0.229 |
| Rwork | 0.225 |
| R-free | 0.29500 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.722 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.900 | 2.500 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.044 | 0.530 |
| Number of reflections | 38743 | |
| <I/σ(I)> | 12.91 | 2.4 |
| Completeness [%] | 95.6 | 66.9 |
| Redundancy | 3.7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 293 | PEG 2000, NaCl, citric acid, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
