3CWV
Crystal structure of B-subunit of the DNA gyrase from Myxococcus xanthus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 160.606, 57.799, 119.437 |
| Unit cell angles | 90.00, 127.23, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.950 |
| R-factor | 0.211 |
| Rwork | 0.209 |
| R-free | 0.24700 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.290 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | HKL2Map |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.068 | 0.294 |
| Number of reflections | 62921 | |
| <I/σ(I)> | 24 | 2.64 |
| Completeness [%] | 98.0 | 86.3 |
| Redundancy | 2.5 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 15 % PEG 3350, 0.1 M Magnesium formate dihydrate, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
