3CWO
A beta/alpha-barrel built by the combination of fragments from different folds
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-10-10 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.071 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 108.840, 108.840, 80.410 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.000 | 3.270 |
High resolution limit [Å] | 3.100 | 3.100 |
Rmerge | 0.093 | 0.549 |
Number of reflections | 7947 | |
<I/σ(I)> | 18.5 | 2.4 |
Completeness [%] | 92.3 | 94.8 |
Redundancy | 13.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1 M 2-(N-morpholino)ethanesulfonic acid (MES) at pH 6.5 with 1.6 M ammonium sulphate and 10% dioxane (v/v), VAPOR DIFFUSION, HANGING DROP, temperature 293K |