3CWG
Unphosphorylated mouse STAT3 core fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-29 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.115891 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 254.779, 254.779, 123.781 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 3.050 |
| Rwork | 0.250 |
| R-free | 0.26850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bg1 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.477 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.160 |
| High resolution limit [Å] | 3.050 | 3.050 |
| Rmerge | 0.113 | 0.447 |
| Number of reflections | 84197 | |
| <I/σ(I)> | 11.1 | 2.1 |
| Completeness [%] | 93.4 | 70.6 |
| Redundancy | 3 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.7 | 277 | 0.1M Bis-tris, 0.5M ammonium sulfate, 12% PEG3350, 0.1mM KCl, 0.05M NaI, pH 5.7, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
