3CWC
Crystal structure of putative glycerate kinase 2 from Salmonella typhimurium LT2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-30 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97920 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.733, 59.549, 103.605 |
| Unit cell angles | 90.00, 106.01, 90.00 |
Refinement procedure
| Resolution | 49.810 - 2.230 |
| R-factor | 0.191 |
| Rwork | 0.188 |
| R-free | 0.23700 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.547 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.810 | 2.270 |
| High resolution limit [Å] | 2.230 | 2.230 |
| Rmerge | 0.075 | 0.622 |
| Number of reflections | 36765 | |
| <I/σ(I)> | 31.4 | 2.09 |
| Completeness [%] | 99.9 | 98.4 |
| Redundancy | 5.5 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 287 | 10% PEG 3000, 0.1 M Phosphate-citrate buffer, 0.2 M Sodium chloride, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 287K |
