3CW5
E. coli Initiator tRNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-3 |
Synchrotron site | ESRF |
Beamline | ID14-3 |
Temperature [K] | 100 |
Collection date | 2006-11-25 |
Wavelength(s) | 0.931 |
Spacegroup name | P 64 2 2 |
Unit cell lengths | 93.790, 93.790, 219.360 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.918 - 3.100 |
R-factor | 0.244 |
Rwork | 0.241 |
R-free | 0.27100 |
Starting model (for MR) | E. coli tRNAfMet from 2FMT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.735 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.2.25) |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 39.918 | 76.250 | 3.270 |
High resolution limit [Å] | 3.100 | 9.800 | 3.100 |
Rmerge | 0.099 | 0.053 | 0.476 |
Total number of observations | 3185 | 2894 | |
Number of reflections | 10063 | ||
<I/σ(I)> | 3.8 | 5.2 | 1.5 |
Completeness [%] | 91.4 | 98.4 | 59.8 |
Redundancy | 7.9 | 7.5 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 4.6 | 292 | 2.0 M Ammonium Sulphate, pH 4.6, vapor diffusion, temperature 292K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | (NH4)2SO4 | ||
2 | 1 | 2 | (NH4)2SO4 |