3CVQ
Structure of Peroxisomal Targeting Signal 1 (PTS1) binding domain of Trypanosoma brucei Peroxin 5 (TbPEX5)complexed to PTS1 peptide (7-SKL)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-08-07 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 68.794, 68.794, 230.232 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.620 - 3.010 |
| R-factor | 0.275 |
| Rwork | 0.273 |
| R-free | 0.32100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB CODE 1FCH |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.487 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 11776 | |
| <I/σ(I)> | 22.7 | 2.6 |
| Completeness [%] | 99.9 | 99.5 |
| Redundancy | 8 | 8.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.8 | 298 | 2.3M Potassium acetate, 0.1M sodium citrate monohydrate, pH 4.8 - 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 298KK, pH 4.80 |
