3CVO

Crystal structure of a methyltransferase-like protein (spo2022) from silicibacter pomeroyi dss-3 at 1.80 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2008-02-28
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91162, 0.97914, 0.97932
Spacegroup name	C 1 2 1
Unit cell lengths	173.391, 61.653, 96.323
Unit cell angles	90.00, 97.45, 90.00

Refinement procedure

Resolution	29.630 - 1.800
R-factor	0.17
Rwork	0.168
R-free	0.19400
Structure solution method	MAD
RMSD bond length	0.016
RMSD bond angle	1.592
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	REFMAC (5.4.0069)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.630	29.630	1.850
High resolution limit [Å]	1.800	8.050	1.800
Rmerge	0.081	0.044	0.648
Total number of observations		3266	21762
Number of reflections	93599
<I/σ(I)>	6.5	13.4	1
Completeness [%]	99.9	97.7	99.8
Redundancy	3.2	3	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.9	277	NANODROP, 2.1M Ammonium sulfate, 1.5% PEG 400, 15.0% Glycerol, 0.1M HEPES pH 6.9, VAPOR DIFFUSION, SITTING DROP, temperature 277K