3CVN
Structure of Peroxisomal Targeting Signal 1 (PTS1) binding domain of Trypanosoma brucei Peroxin 5 (TbPEX5)complexed to T. brucei Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) PTS1 peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-12-07 |
Detector | RIGAKU SATURN 944 |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 44.619, 66.178, 51.979 |
Unit cell angles | 90.00, 104.55, 90.00 |
Refinement procedure
Resolution | 40.060 - 2.000 |
R-factor | 0.196 |
Rwork | 0.192 |
R-free | 0.26500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB code 1FCH |
RMSD bond length | 0.013 |
RMSD bond angle | 1.333 |
Data reduction software | d*TREK |
Data scaling software | d*TREK (9.7L) |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.190 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 19415 | |
<I/σ(I)> | 8.4 | 2.2 |
Completeness [%] | 97.6 | 81.3 |
Redundancy | 3.48 | 2.32 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 2.3M Potassium acetate, 0.1M sodium citrate monohydrate, pH 4.8 - 5.0, vapor diffusion, sitting drop, temperature 298KK |