3CVF

Crystal Structure of the carboxy terminus of Homer3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X26C
Synchrotron site	NSLS
Beamline	X26C
Temperature [K]	100
Detector technology	CCD
Collection date	2006-11-09
Detector	Quantum-4 ADSC
Wavelength(s)	0.9795
Spacegroup name	P 65
Unit cell lengths	172.029, 172.029, 66.446
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.730 - 2.900
R-factor	0.25391
Rwork	0.252
R-free	0.28698
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3cve
RMSD bond length	0.025
RMSD bond angle	2.312
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.000
High resolution limit [Å]	2.900	6.240	2.900
Rmerge	0.078	0.044	0.255
Number of reflections	25036
<I/σ(I)>	14.5
Completeness [%]	99.4	98.1	98.1
Redundancy	7.2	8.2	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	hanging drop	7.6	297	0.1 M Tris, 4.2 M NaCl, 10% glycerol, pH 7.6, hanging drop, temperature 297K