3CVE
Crystal Structure of the carboxy terminus of Homer1
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X26C |
| Synchrotron site | NSLS |
| Beamline | X26C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-08-12 |
| Detector | Quantum-4 ADSC |
| Wavelength(s) | 0.9794,0.9801,0.9600 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.014, 62.508, 57.526 |
| Unit cell angles | 90.00, 102.14, 90.00 |
Refinement procedure
| Resolution | 7.970 - 1.750 |
| R-factor | 0.22 |
| Rwork | 0.216 |
| R-free | 0.28900 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.302 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.810 |
| High resolution limit [Å] | 1.750 | 3.770 | 1.750 |
| Rmerge | 0.058 | 0.050 | 0.196 |
| Number of reflections | 26705 | ||
| <I/σ(I)> | 19.8 | ||
| Completeness [%] | 97.9 | 90.7 | 96.4 |
| Redundancy | 3.4 | 3.2 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop | 6 | 297 | 0.1 M MES, 0.1 M ammonium sulfate, 16% PEG1000, 10% glycerol, pH 6.0, hanging drop, temperature 297K |
