3CUY
Crystal Structure of GumK mutant D157A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | LNLS BEAMLINE D03B-MX1 |
Synchrotron site | LNLS |
Beamline | D03B-MX1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-04-01 |
Detector | MARRESEARCH |
Wavelength(s) | 1.433 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 121.726, 121.726, 172.557 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 14.990 - 2.300 |
R-factor | 0.184 |
Rwork | 0.182 |
R-free | 0.22500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2hy7 |
RMSD bond length | 0.018 |
RMSD bond angle | 1.622 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.2.5) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.220 | 2.420 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.171 | 0.281 |
Number of reflections | 33977 | |
<I/σ(I)> | 3.1 | 6.7 |
Completeness [%] | 99.5 | 100 |
Redundancy | 7.6 | 8.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | hanging drop | 8.2 | 293 | 0.1 M Tris-HCl, 35% (w/v) PEG 3350, 0.2 M Li2SO4, 0.1 M LiCl, pH 8.2, hanging drop, temperature 293K |