3CUW
Crystal structure of glycogen phosphorylase b in complex with N-(-D-glucopyranosyl)-N'-(2-naphthyl)oxamides
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2006-01-01 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.8063 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 128.418, 128.418, 116.353 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 21.260 - 2.000 |
| R-factor | 0.19282 |
| Rwork | 0.192 |
| R-free | 0.21649 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1hlf |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.031 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 65843 | |
| <I/σ(I)> | 17.1 | 5.1 |
| Completeness [%] | 99.5 | 99.4 |
| Redundancy | 5.3 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | SMALL TUBES | 6.7 | 287 | BES, EDTA, pH6.7, SMALL TUBES, temperature 287K |
