3CUU
Crystal structure of glycogen phosphorylase b in complex with N-(-D-glucopyranosyl)-N'-(2-naphthyl)oxamides
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX10.1 |
| Synchrotron site | SRS |
| Beamline | PX10.1 |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2007-11-16 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.04498 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 128.200, 128.200, 116.030 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.060 - 2.300 |
| R-factor | 0.18222 |
| Rwork | 0.180 |
| R-free | 0.21753 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1hlf |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.066 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.700 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 42548 | |
| <I/σ(I)> | 11.4 | 4.8 |
| Completeness [%] | 98.4 | 98.5 |
| Redundancy | 2.6 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | SMALL TUBES | 6.7 | 287 | BES, EDTA, pH6.7, SMALL TUBES, temperature 287K |
