3CU9
High resolution crystal structure of 1,5-alpha-L-arabinanase from Geobacillus Stearothermophilus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-27 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.978578 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 86.141, 89.579, 76.041 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.060 |
| R-factor | 0.1472 |
| Rwork | 0.147 |
| R-free | 0.17610 |
| Structure solution method | AB INITIO |
| Starting model (for MR) | 1wl7 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 0.031 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELX |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.080 |
| High resolution limit [Å] | 1.060 | 1.060 |
| Rmerge | 0.096 | 0.258 |
| Number of reflections | 130844 | |
| <I/σ(I)> | 16.7 | |
| Completeness [%] | 98.5 | 77.7 |
| Redundancy | 14.6 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 1.7M Lithium Sulfate, 0.1M Tris buffer pH 7.5, 15% (v/v) Glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
