3CU5
Crystal structure of a two component transcriptional regulator AraC from Clostridium phytofermentans ISDg
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-04-04 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.97980 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 34.326, 87.038, 52.697 |
Unit cell angles | 90.00, 106.22, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.600 |
R-factor | 0.189 |
Rwork | 0.183 |
R-free | 0.30000 |
Structure solution method | SAD |
RMSD bond length | 0.018 |
RMSD bond angle | 2.098 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELX |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.590 |
High resolution limit [Å] | 2.500 | 5.380 | 2.500 |
Rmerge | 0.101 | 0.061 | 0.407 |
Number of reflections | 16975 | ||
<I/σ(I)> | 7.7 | ||
Completeness [%] | 85.8 | 99.3 | 36.4 |
Redundancy | 3.3 | 3.8 | 1.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 294 | 1.8 M Tri-ammonium citrate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 294K |