3CU2
Crystal structure of ribulose-5-phosphate 3-epimerase (YP_718263.1) from Haemophilus somnus 129PT at 1.91 A resolution
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-04 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.91837, 0.97922, 0.97840 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 85.010, 85.010, 140.160 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.916 - 1.910 |
| R-factor | 0.168 |
| Rwork | 0.166 |
| R-free | 0.20500 |
| Structure solution method | MAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.611 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.4.0067) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 28.916 | 28.916 | 1.980 |
| High resolution limit [Å] | 1.910 | 4.100 | 1.910 |
| Rmerge | 0.120 | 0.032 | 0.764 |
| Number of reflections | 46203 | 8884 | 9090 |
| <I/σ(I)> | 7.72 | 25.9 | 1.4 |
| Completeness [%] | 99.8 | 99.5 | 99.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.17 | 277 | NANODROP, 16.0% PEG 8000, 0.167M Calcium acetate, 0.1M MES pH 6.17, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
