3CTW

Crystal Structure of RcdA from Caulobacter crescentus CB15

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Detector technology	CCD
Collection date	2007-08-26
Detector	ADSC Q315
Wavelength(s)	0.97957,1.01986,0.97972
Spacegroup name	P 43
Unit cell lengths	75.458, 75.458, 81.419
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.630 - 2.900
R-factor	0.276
Rwork	0.274
R-free	0.31200
Structure solution method	MAD
RMSD bond length	0.011
RMSD bond angle	1.645
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELX
Refinement software	REFMAC (5.4.0067)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.900
High resolution limit [Å]	2.800	6.030	2.800
Rmerge	0.099	0.039	0.458
Number of reflections	10955
<I/σ(I)>	7.5
Completeness [%]	96.9	100	75.4
Redundancy	4.8	4.8	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	100mM HEPES, 1.75M Formate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	100mM HEPES, 1.75M Formate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K