3CTP
Crystal structure of periplasmic binding protein/LacI transcriptional regulator from Alkaliphilus metalliredigens QYMF complexed with D-xylulofuranose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-04 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97980 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.172, 61.903, 74.362 |
| Unit cell angles | 90.00, 104.81, 90.00 |
Refinement procedure
| Resolution | 9.990 - 1.410 |
| R-factor | 0.183 |
| Rwork | 0.182 |
| R-free | 0.20700 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.510 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.460 |
| High resolution limit [Å] | 1.410 | 3.040 | 1.410 |
| Rmerge | 0.076 | 0.049 | |
| Number of reflections | 206130 | ||
| <I/σ(I)> | 7.8 | ||
| Completeness [%] | 96.6 | 99.4 | 72.2 |
| Redundancy | 3.5 | 3.9 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 294 | 0.1M Hepes pH 7.5, 25% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
