3CT4
Structure of Dha-kinase subunit DhaK from L. Lactis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X12 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X12 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-15 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.79997 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 107.802, 107.802, 142.900 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.372 - 2.498 |
| R-factor | 0.2038 |
| Rwork | 0.200 |
| R-free | 0.26700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1uod |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.913 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.530 |
| High resolution limit [Å] | 2.440 | 2.440 |
| Rmerge | 0.090 | 0.428 |
| Number of reflections | 36223 | |
| <I/σ(I)> | 9.4 | |
| Completeness [%] | 97.7 | 78 |
| Redundancy | 6.5 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 0.05 M TRIS/HCl, 0.2 M NH4Cl, 0.01 M CaCl2, 30% PEG 4000, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
