3CT4
Structure of Dha-kinase subunit DhaK from L. Lactis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X12 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X12 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-04-15 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.79997 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 107.802, 107.802, 142.900 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 44.372 - 2.498 |
R-factor | 0.2038 |
Rwork | 0.200 |
R-free | 0.26700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1uod |
RMSD bond length | 0.006 |
RMSD bond angle | 0.913 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.530 |
High resolution limit [Å] | 2.440 | 2.440 |
Rmerge | 0.090 | 0.428 |
Number of reflections | 36223 | |
<I/σ(I)> | 9.4 | |
Completeness [%] | 97.7 | 78 |
Redundancy | 6.5 | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 0.05 M TRIS/HCl, 0.2 M NH4Cl, 0.01 M CaCl2, 30% PEG 4000, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |