3CSZ
Crystal and cryoEM structural studies of a cell wall degrading enzyme in the bacteriophage phi29 tail
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-03-04 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 30.876, 69.350, 70.728 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 21.970 - 1.800 |
| R-factor | 0.18715 |
| Rwork | 0.183 |
| R-free | 0.22522 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.389 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.053 | 0.342 |
| Number of reflections | 14346 | |
| <I/σ(I)> | 32.1 | 6.3 |
| Completeness [%] | 98.0 | 96 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 20% PEG4000, 100mM Tris-HCl, 10% glyerol, 1.5 mg/ml (N-acetylglucosamine)3 (NAG)3, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
