3CSX
Structural characterization of a protein in the DUF683 family- crystal structure of cce_0567 from the cyanobacterium Cyanothece 51142.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97935 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 29.436, 38.381, 62.643 |
| Unit cell angles | 90.00, 96.70, 90.00 |
Refinement procedure
| Resolution | 29.240 - 1.840 |
| R-factor | 0.21994 |
| Rwork | 0.216 |
| R-free | 0.29387 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.506 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.920 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Number of reflections | 11230 | |
| Completeness [%] | 92.0 | 52 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 295 | 2 uL Protein (~2 mg/mL protein in 500 mM NaCl, 20 mM TrisHCl, 1.0 mM DTT, pH 7.2) plus 2 uL Precipatant (30% w/v PEG 1500, 1 mM NiCl2)., VAPOR DIFFUSION, HANGING DROP, temperature 295K |
