3CSO
HCV Polymerase in complex with a 1,5 Benzodiazepine inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-01-10 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 105.962, 108.224, 133.304 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 83.050 - 2.710 |
| R-factor | 0.18331 |
| Rwork | 0.180 |
| R-free | 0.23943 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.302 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | Mol. (Rep.) |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 83.050 | 2.800 |
| High resolution limit [Å] | 2.710 | 2.710 |
| Rmerge | 0.162 | 0.614 |
| Number of reflections | 42311 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 4.1 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.75 | 293 | 5-8% PEG 6000, 100 mM Mg-salts, pH 6.75, VAPOR DIFFUSION, temperature 293K |
