3CSN
Structure of the Serratia marcescens hemophore receptor HasR in complex with its hemophore HasA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2007-10-27 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | F 2 2 2 |
| Unit cell lengths | 157.650, 164.740, 597.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.430 - 3.000 |
| R-factor | 0.219 |
| Rwork | 0.217 |
| R-free | 0.24400 |
| Structure solution method | Difference fourier |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.128 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.400 | 3.200 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.145 | 0.749 |
| Number of reflections | 77295 | |
| <I/σ(I)> | 9.79 | 2.15 |
| Completeness [%] | 99.6 | 98.6 |
| Redundancy | 10.46 | 5.13 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 291 | 2 M NaCl, 100 mM Tris, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
